Drug detection times

Drug drivers are on notice this year, with several state governments in Australia increasing roadside drug testing and toughening up measures against drug taking motorists in a bid to lower road incidents. With that, many are concerned about drug detection times not only in the workplace, but also when it comes to roadside drug testing detection times.

Previously, roadside drug tests only picked up ecstasy, cannabis and speed. This year, the big change is the addition of cocaine drug tests to roadside testing – part of a push by authorities to reduce road deaths which soared to a eight-year-high of 392 in 2017 (in NSW).

Drug testing has been used for years in law enforcement, medical purposes as well as in the enforcement of workplace ethics. As drug testing is generally carried out to achieve a desired outcome or to address an identified risk, it does not occur in a vacuum. There are well laid out policies, regulations and procedures that have to be followed during the drug testing process.

We discuss some basics of understanding drug detection times including drug screening in the workplace and during roadside drug tests, drug test cut-off levels, the standards that govern these cut-off levels, and the different drug test detection times for different substances.

Drug detection times

Drug Screening

Drug tests carried out at workplaces and at roadside drug testing are actually rapid drug screening tests designed to quickly identify a sample as either negative or presumptive positive. Screening methods are based on established imunoassay technologies, which utilise antibodies as reagents to measure the amount of a particular drug or drug group in the sample.

As oral and urine drug testing kits are screening tools, all presumptive positives require a confirmation test, which provides qualitative results of drugs and/or drug metabolites present in the sample. Confirmation results are expressed as positive or negative and are legally defensible. Usual methods of confirmation testing involve gas chromatography or liquid chromatography – highly specific and sensitive analytical methodology that can identify specific drugs and their quantities in the sample.

Drug Test Cut-Off Limits

The key concept in drug testing kits is the application of cut-off levels, which is the point that separates a test result as being either positive or negative. A cut-off limit is the minimum concentration of drugs or metabolites that must be present in specimens before the drug test result can be reported as positive.

In drug screening tests, a cut-off limit is chosen that will optimise drug detection but minimise the number of false positive results.

It is also important to note that a negative result on a drug screening test doesn’t mean that it is drug free; it might contain a drug at a concentration that is lower than the defined cut-off.

On the other hand, a screen positive or presumptive positive drug test result merely shows a response, which is usually because a drug is present. However, it does not indicate recency, frequency, or amount of use, nor can it be correlated to any degree of impairment.

Australian Drug Test Cut-Off Levels

In Australia, the cut-off levels of the commonly abused drugs have been defined under the Australian AS/NZS 4308:2008 Standards (urine testing), the AS 4760:2006 Standards (saliva testing) and the AS 3547:1997 Standards (alcohol breath testing).

These standards were established to offer guidance on the procedures used in drug testing including specimen collection, detection and quantification of the specific drugs in urine and saliva tests.

The tables below show the Australian Standard cut-off levels for the detection of drugs in urine and saliva tests:

Cut-Off Levels under the AS 4308 Standards (Urine)

Code Drug Tested Cut-Off Level
THC Cannabis Metabolites 50 µg/L
COC Cocaine Metabolites 300 µg/L
AMP Amphetamines 300 µg/L
MET Methamphetamines 300 µg/L
BZO Benzodiazepines 200 µg/L
OPI Opiates 300 µg/L

Cut-Off Levels under the AS 4760:2006 Standards (Saliva)

Code Drug Tested Cut-Off Level
THC Cannabis 25 µg/L
COC Cocaine and Metabolites 50 µg/L
AMP Amphetamines 50 µg/L
OPI Opiates 50 µg/L


Window of Drug Detection Times

Drugs will not stay in the body system forever. Different types of drugs are metabolised by the body at different rates, and the duration over which a particular class of drug will stay in the body also varies from one person to another.

The factors affecting the drug detection times include the amount of drugs that was consumed by the person, mode of drug use, body size of the drug user and the efficiency with which a person’s body process the drugs.

The window of detection for drug detection times is the time that a drug can be detected in a biological sample above a specified cut-off for the test being performed. There is a period of time immediately before and after this detection window when the drug will be present in the sample but at a level below the cut-off.

Window of Detection

A visual representation of a cut-off level and window of detection (source)

 Tests are designed to be drug or drug group specific and the cut-off level has been determined to optimise detection without giving false positives.

Drugs are detected in oral fluid either from direct deposition in the mouth or by transfer from the blood stream following ingestion and absorption. Any drugs present in the bloodstream are metabolised in the liver before being excreted in the urine. This results in drugs appearing in the urine later than oral fluid. Therefore, drugs are detectable for a significantly longer time in urine than they are in oral fluid.

The table below provides a general estimate of the drug detection times for the various classes of drugs:-

Drug Detection Times in Oral Fluid

The shortest window of detection is found with oral fluid.

Drug Detection Window
Cocaine up to 24 hours
Benzodiazepines up to 24 hours
Cannabinoids (THC) up to 24 hours
Methamphetamines up to 24 hours
up to 1-2 days
up to 24 hours
up to 1-2 days
Amphetamine up to 1-2 days
Buprenorphine up to 1-2 days
Ketamine up to 1-2 days
Methadone up to 1-2 days

Drug Detection Times in Urine

Drugs can be detected in urine for longer, and in consequence the window of detection for some drugs can be affected by whether their effects are short or long acting.

Drug Detection Window
Ultra short acting
Short acting
Intermediate acting
up to 12 hours
up to 1 day
up to 2-4 days
up to 7 days 
Shorter acting
up to 1-2 days
up to 7 days 
Methadone up to 1-2 days
Amphetamines up to 1-3 days
Methamphetamines up to 1-3 days
Buprenorphine/Subutex Analgesic
Therapeutic dose
Maintenance dose
up to 1-3 days
up to 10-12 days
Cannabinoids (THC) up to 1-4 days
Cocaine up to 2-3 days
Opiates up to 2-3 days
Ketamine up to 3-5 days
Tramadol up to 3-5 days


Many drug testing kits detect and test for the most commonly abused “street” drugs, which are:

  • Opiate
  • Amphetamines
  • Methamphetamines
  • Cocaine
  • Cannabis/Marijuana

However, there are many more drug testing kits that extend up to other classes of drugs such as the various prescription drugs that may be abused by users. These drugs include:

  • Benzodiazepines
  • Ecstasy
  • Narcotic prescription drugs such as Methadone and Oxycodone
  • Barbiturates

Note that different drug testing kits may test for different drugs and have different cut-off levels than those prescribed by Australian Standards. Some may also test for additional drugs or metabolites, such as the DrugSense DSO7 saliva drug testing kit which tests for MDMA separately, and the DrugSense DSU9 urine drug testing kit which can provide a BAC reading for alcohol in the urine sample.

For information and assistance with drug testing Australia workplace employees, be sure to talk to our workplace drug testing experts on 1300 800 200 (AU) or +613 8899 6900 (International) for a free consultation.



Procedures for specimen collection and the detection and quantitation of drugs of abuse in urine, https://www.saiglobal.com/PDFTemp/Previews/OSH/AS/AS4000/4300/4308-2008.pdf

Procedures for specimen collection and the detection and quantitation of drugs in oral fluid, https://www.saiglobal.com/PDFTemp/Previews/OSH/AS/AS4000/4700/4760-2006.pdf

Windows of Detection, https://www.aleretoxicology.co.uk/en/home/support/testing-explained/windows-of-detection.html

Cut-off Levels, https://www.aleretoxicology.co.uk/en/home/support/testing-explained/cut-off-levels.html


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